By Greg Arnold, DC, CSCS, December 8, 2008, abstracted from “The dietary phytochemical indole-3-carbinol is a natural elastase enzymatic inhibitor that disrupts cyclin E protein processing“, printed online December 8, 2008 in the Proceedings of the National Academy of Sciences
Cruciferous vegetables, which include broccoli, cabbage, cauliflower, Brussels sprouts, turnip, and radish (1) owe their health-promoting properties to antioxidants called indoles (2). One such indole is called Indole-3-Carbinol (I3C), which benefits overall cell health (3), infant health (4), detoxification (5), bladder health (6), and heart health (7). Another antioxidant called sulfurophane has been shown to help prostate health (8).
Now a new study (9) has found how I3C is able to help with breast cell health. Previous research has shown that cruciferous vegetable consumption decreases the risk of reproductive tissue cancer (10). In the new study, researchers identified the first target of I3C in breast cancer to be a protein called elastase, which is highly expressed in human breast cancers and has been implicated in tumor progression (11). Specifically, the researchers treated late stage breast cancer cells (MDA-MB-231 cells) with 100 micromoles of I3C, resulting in decreased production of inflammatory cells that promote cancer progression.
The importance of this study is not only that breast cancer is second only to lung cancer as the biggest cancer killer of American women. It caused an estimated 40,580 deaths in 2004 (12) and accounted for up to 25% of the $157 billion in cancer costs in the U.S. in 2001 (13). “A critical challenge” in controlling breast cancer is finding therapies that can control breast cancer progression with reduced side effects, especially during prolonged treatments. They may now have their therapy in I3C and their therapy target in elastase.
For the researchers, “our results demonstrate that elastase is the first identified specific target protein for I3C and…implicates the potential use of this indole, or related compounds, in targeted therapies of human breast cancers.” In addition to I3C, other ways to help maintain breast health include include folic acid (14), resveratrol (15), kelp (16), vitamin E (17), black cohosh (18), soy (19), curcumin (20), and lignan (studies in 2006 (21) and 2007 (22)).
Greg Arnold is a Chiropractic Physician practicing in Danville, CA. You can contact Dr. Arnold directly by emailing him at mailto:PitchingDoc@msn.com or visiting his web site at www.CompleteChiropracticHealthcare.com
1. Keck AS, Finley JW. Cruciferous vegetables: cancer protective mechanisms of glucosinolate hydrolysis products and selenium. Integr Cancer Ther. 2004;3:5–12
2. Pedras MS, Nycholat CM, Montaut S, Xu Y, Khan AQ. Chemical defenses of crucifers: elicitation and metabolism of phytoalexins and indole-3-acetonitrile in brown mustard and turnip. Phytochemistry 2002;59:611–25.
3. Arora A. Modulation of vinca-alkaloid-induced P-glycoprotein expression by indole 3-carbinol. Cancer Letters 2003; 189: 167-173
4. Williams DE. Indole-3-carbinol in the maternal diet provides chemoprotection for the fetus against transplacental carcinogenesis by the polycyclic aromatic hydrocarbon dibenzo[a,l]pyrene. Carcinogenesis Advance Access published on May 16, 2006 Carcinogenesis 2006 27: 2116-2123
5. Finley JW. The antioxidant responsive element (ARE) may explain the protective effects of cruciferous vegetables on cancer. Nutr Rev. 2003 Jul;61(7):250-4. Review
6. Munday R. Inhibition of Urinary Bladder Carcinogenesis by Broccoli Sprouts. Cancer Res 2008 68: 1593-1600. Published Online First February 29, 2008. doi: 10.1158/0008-5472.CAN-07-5009
7. Maiyoh GK. Cruciferous Indole-3-Carbinol Inhibits Apolipoprotein B Secretion in HepG2 Cells. J. Nutr. 137: 2185–2189, 2007.
8. Wang, L., et al., Targeting cell cycle machinery as a molecular mechanism of sulforaphane in prostate cancer prevention. Int J Oncol, 2004. 24(1): p. 187-92
9. HigdonJV,DelageB, WilliamsDE,DashwoodRH(2007) Cruciferous vegetables and human cancer risk: Epidemiologic evidence and mechanistic basis. Pharmacol Res 55:224–236.
10. Nguyen HH. The dietary phytochemical indole-3-carbinol is a natural elastase enzymatic inhibitor that disrupts cyclin E protein processing. PNAS published online before print December 8, 2008, doi:10.1073/pnas.080658110
11. Sato T, et al. (2006) Neutrophil elastase and cancer. Surg Oncol 15:217–222.
12. "2004/2005 Fact Sheet Cancer Registries: The Foundation for Cancer Prevention and Control" posted on the CDC website www.cdc.gov/cancer
13. Radice D. Breast cancer management: quality-of-life and cost considerations. Pharmacoeconomics 2003;21(6):383-96
14. Ericson U. High folate intake is associated with lower breast cancer incidence in postmenopausal women in the Malmö Diet and Cancer cohort Am. J. Clinical Nutrition, Aug 2007; 86: 434 – 443
15. Tang FY. Resveratrol inhibits heregulin-â1-mediated matrix metalloproteinase-9 expression and cell invasion in human breast ancer cells. Jou Nutr Biochem 2007. In Press, Corrected Proof, Available online 24 July 2007
16. Skibola CF. Brown kelp modulates endocrine hormones in female sprague-dawley rats and in human luteinized granulosa cells. J Nutr 2005; 135(2): 296-300
17. Dorigochoo T. Vitamin supplement use and risk for breast cancer: the Shanghai Breast Cancer Study. Breast Cancer Res Treatment 2007. Printed online DOI 10.1007/s10549-007-9772-8
18. Einbond LS. Growth inhibitory activity of extracts and compounds from Cimicifuga species on human breast cancer cells. Phytomedicine 2007. In Press, Corrected Proof, Available online 5 November 2007
19. Lampe JW. Plasma Isoflavones and Fibrocystic Breast Conditions and Breast Cancer Among Women in Shanghai, China. Cancer Epidemiol Biomarkers Prev 2007 16: 2579-2586 doi: 10.1158/1055-9965.EPI-07-0368
20. Rattanamongkolgul, S., K. Muir, et al. (2002). "Diet, energy intake and breast cancer risk in an Asian country." IARC Sci Publ 156: 543-5
21. Piller R. Plasma enterolactone and genistein and the risk of premenopausal breast cancer. Eur J Cancer Prev. 2006 Jun;15(3):225-32
22. Touillaud MS. Dietary Lignan Intake and Postmenopausal Breast Cancer Risk by Estrogen and Progesterone Receptor Status. J. Natl. Cancer Inst. 2007 99: 475-486; doi:10.1093/jnci/djk096